Coding

Part:BBa_K1690005

Designed by: Anna Sosnova, Pavel Zach   Group: iGEM15_Czech_Republic   (2015-09-17)


Pheromone receptor (C. parapsilosis)

This is a coding sequence of C. parapsilosis mating pheromone receptor. Cells with this receptor being expressed react to the C. parapsilosis mating pheromone by the activation of the pheromone mating pathway.

The coding sequence was codon optimized for the expression in S. cerevisiae. C-terminal was replaced by a C-terminal from C. albicans for more efficient coupling to the mating pathway in S. cerevisiae.

Detailed description can be obtained from the [http://2015.igem.org/Team:Czech_Republic/Project/Synthetic_signals_and_receptors#Receptors iGEM 2015 Czech Republic wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 280
    Illegal BglII site found at 1085
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None